However, Morris water maze studies with mice have principally been performed using adult animals, which preclude studies of critical neurodevelopmental periods when the cellular and molecular substrates of learning and memory are formed. A widely used method for assessing these cognitive processes in mice is the Morris water maze, a classic test for examining spatial learning and memory. 2016, 6, 22429.Mouse models have been indispensable for elucidating normal and pathological processes that influence learning and memory. NO 2 inhalation promotes Alzheimer's disease-like progression: cyclooxygenase-2-derived prostaglandin e 2 modulation and monoacylglycerol lipase inhibition-targeted medication. If you are interested, click the links for more detailed description.įor more information, please contact us or send us an inquiry. Moreover, new behavioral tests of cognition are constantly developed and validated.Īs an undisputed specialist in neurological disease drug development, Creative Biolabs has an extensive range of rodent neurological disease models, which are widely used for drug efficacy studies combined with different behavioral tests. 2016)Ĭreative Biolabs also conducts other behavioral tests for cognition and memory assessment in rodents:Ĭreative Biolabs provides highly customized behavioral tests to suit specific scientific needs of our clients.
(c) Percentages of time stayed in the target quadrant. (b) The number of times crossing the target zone. (a) Learning curve for 5 days of invisible training to find the hidden platform in the Morris water maze. Visible platform tests can recognize gross visual deficiencies that might confound interpretation of results obtained from standard Morris water task testing.īoth learning and re-learning experiments can be done by changing the position of the platform.įig.1 NO 2 inhalation deteriorates spatial learning and memory in C57BL/6J mice. Non-mnemonic behaviors or strategies can be described, and motor or motivational insufficiencies can be recognized through the use of video tracking strategies. The beauty of the task is that the water prevents the animals from using their proprioception (number of steps) to assess the traversed distance and forces them to rely on visual landmarks of the environment.īoth learning and retrieval processes can be evaluated through the use of training as well as a probe or transfer trials. Moreover, the swim pattern is monitored by a video-tracking system and the time in the target quadrant or the former platform area is calculated.
Any maze latency to esacpe trial#
After the escape latency to the hidden platform reaches a plateau, the spatial memory is separately assessed in a probe trial without the platform, which is usually done 24 h after the last acquisition trial. Before the testing session starts, animals are trained for 3-5 days with 3-5 daily trials. The crux of the task is that the animal is released from four different sites, so that an egocentric response (‘straight ahead and then a gentle left turn') will not help to find the platform in more than one of the starting locations. The only spatial cues are mainly the visual cues exterior of the tank. The platform offers no local cues to direct the escape behavior of the animal. When the animal is released into the water, it will swim around the pool to look for the platform to escape from the water. The apparatus consists of a water-filled pool with a hidden escape platform beneath the surface of the water. It is based on the rodent's (rats or mice) aversion to the water environment. The Morris water task has become a gold standard task for special learning and memory. By recording the escape latency (time to find the hidden platform form the starting point) and time spent in the target quadrant studying, we can assess their ability to learn and remember the location of the platform. Creative Biolabs performs this test to assess the effects of test compounds on both learning and memory. Morris water task is a test widely used and established by behavioral physiologist and pharmacologist to evaluate and compare learning and memory in rodents. Pharmacology and Pharmacodynamics Studies.Developability Assessment of Biopharmaceutical Candidates.Small-Angle X-ray and Neutron Scattering.Nuclear Magnetic Resonance (NMR) Spectroscopy.Natural Bioactive Compound Target Identification.In Silico Functional Analysis for Fungi.Computer-Aided Target Identification and Validation.Identification of Biomarkers by ORFeome Phage Display.Target Discovery for Cancer Immunotherapy.
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